Keywords
p53, Immunohistochemistry, Mutant p53, Tumor Suppressor
Methods
The techniques of nuclear classification algorithm is used to detect the nuclei in morphological form and size and classify them as positive or negative according to pixel colorization and intensity. Also, tile extraction, color deconvolution, morphological filters, threshold value method,and cell segmentation algorithms are used to develop p53 analysis.
The analysis algorithm contains H-DAB colour deconvolution method. To detect negative nuclei H channel, and to detect and classify brown nuclei DAB channel are used. The positive nuclei are classified into three groups according to given threshold values by the user before running the analysis.
References
Muller PA, Vousden KH. Mutant p53 in cancer: new functions and therapeutic opportunities. Cancer Cell. 2014;25(3):304‐317. doi:10.1016/j.ccr.2014.01.021.